[HTML][HTML] The Inhibitor of Apoptosis, cIAP2, Functions as a Ubiquitin-Protein Ligase and Promotes in VitroMonoubiquitination of Caspases 3 and 7
H Huang, CAP Joazeiro, E Bonfoco, S Kamada… - Journal of Biological …, 2000 - ASBMB
The inhibitor of apoptosis, cIAP2, contains a putative Ring finger motif at the C terminus.
Using in vitro ubiquitination assays, we found that the Ring finger of cIAP2 alone possesses
intrinsic ubiquitin ligase activity and promotes substrate-independent ubiquitination. It also
promotes ubiquitination of caspases 3 and 7 but not caspase-1. The Ring fingers of c-Cbl
and Apc11 failed to promote caspase-7 ubiquitination, suggesting that the Ring finger of
cIAP2 itself is involved in substrate recognition.
Using in vitro ubiquitination assays, we found that the Ring finger of cIAP2 alone possesses
intrinsic ubiquitin ligase activity and promotes substrate-independent ubiquitination. It also
promotes ubiquitination of caspases 3 and 7 but not caspase-1. The Ring fingers of c-Cbl
and Apc11 failed to promote caspase-7 ubiquitination, suggesting that the Ring finger of
cIAP2 itself is involved in substrate recognition.