A novel technique for the fluorometric assessment of T lymphocyte antigen specific lysis
ME Sheehy, AB McDermott, SN Furlan… - Journal of …, 2001 - Elsevier
The 51Cr release assay has traditionally been used to investigate effector cell cytotoxic
function against labeled targets, but this method has inherent problems that include hazards
associated with radioactivity, cell labeling and high spontaneous release. Here we describe
a novel flow cytometric assay which addresses and improves upon the problems currently
encountered with the 51Cr release assay. The fluorometric assessment of T lymphocyte
antigen specific lysis (FATAL) assay employs dual staining (PKH-26 and CFSE) to identify …
function against labeled targets, but this method has inherent problems that include hazards
associated with radioactivity, cell labeling and high spontaneous release. Here we describe
a novel flow cytometric assay which addresses and improves upon the problems currently
encountered with the 51Cr release assay. The fluorometric assessment of T lymphocyte
antigen specific lysis (FATAL) assay employs dual staining (PKH-26 and CFSE) to identify …
