Factor XII–independent activation of the bradykinin-forming cascade: Implications for the pathogenesis of hereditary angioedema types I and II
K Joseph, BG Tholanikunnel, A Bygum… - Journal of allergy and …, 2013 - Elsevier
Background We have previously reported that prekallikrein expresses an active site when it
is bound to high-molecular-weight kininogen (HK) and can digest HK to produce bradykinin.
The reaction is stoichiometric and inhibited by C1 inhibitor (C1-INH) or corn trypsin inhibitor.
Addition of heat shock protein 90 leads to conversion of prekallikrein to kallikrein in a zinc-
dependent reaction. Objective Our goal was to determine whether these reactions are
demonstrable in plasma and distinguish them from activation through factor XII. Methods …
is bound to high-molecular-weight kininogen (HK) and can digest HK to produce bradykinin.
The reaction is stoichiometric and inhibited by C1 inhibitor (C1-INH) or corn trypsin inhibitor.
Addition of heat shock protein 90 leads to conversion of prekallikrein to kallikrein in a zinc-
dependent reaction. Objective Our goal was to determine whether these reactions are
demonstrable in plasma and distinguish them from activation through factor XII. Methods …