[HTML][HTML] Enhanced N-glycosylation site analysis of sialoglycopeptides by strong cation exchange prefractionation applied to platelet plasma membranes
U Lewandrowski, RP Zahedi, J Moebius… - Molecular & Cellular …, 2007 - ASBMB
Elucidation of post-translational modifications to proteins, such as glycosylations or
phosphorylations, is one of the major issues concerning ongoing proteomics studies. To
reduce general sample complexity, a necessary prerequisite is specific enrichment of
peptide subsets prior to mass spectrometric sequencing. Regarding analysis of overall N-
glycosylation sites in the past, this has been achieved by several approaches proving to be
more or less complicated and specific. Here we present a novel strategy to target N …
phosphorylations, is one of the major issues concerning ongoing proteomics studies. To
reduce general sample complexity, a necessary prerequisite is specific enrichment of
peptide subsets prior to mass spectrometric sequencing. Regarding analysis of overall N-
glycosylation sites in the past, this has been achieved by several approaches proving to be
more or less complicated and specific. Here we present a novel strategy to target N …