[HTML][HTML] Initial characterization of the glutamate-cysteine ligase modifier subunit Gclm (−/−) knockout mouse: novel model system for a severely compromised oxidative …
YI Yang, MZ Dieter, Y Chen, HG Shertzer… - Journal of Biological …, 2002 - ASBMB
Glutamate-cysteine ligase (GCL) is the rate-limiting enzyme in the GSH biosynthesis
pathway. In higher eukaryotes, this enzyme is a heterodimer comprising a catalytic subunit
(GCLC) and a modifier subunit (GCLM), which change the catalytic characteristics of the
holoenzyme. To define the cellular function of GCLM, we disrupted the mouse Gclm gene to
create a null allele. Gclm (−/−) mice are viable and fertile and have no overt phenotype. In
liver, lung, pancreas, erythrocytes, and plasma, however, GSH levels in Gclm (−/−) mice …
pathway. In higher eukaryotes, this enzyme is a heterodimer comprising a catalytic subunit
(GCLC) and a modifier subunit (GCLM), which change the catalytic characteristics of the
holoenzyme. To define the cellular function of GCLM, we disrupted the mouse Gclm gene to
create a null allele. Gclm (−/−) mice are viable and fertile and have no overt phenotype. In
liver, lung, pancreas, erythrocytes, and plasma, however, GSH levels in Gclm (−/−) mice …