Evaluation of methods of detecting cell reactive oxygen species production for drug screening and cell cycle studies
LM Fan, JM Li - Journal of pharmacological and toxicological methods, 2014 - Elsevier
Intracellular reactive oxygen species (ROS) production is essential to normal cell function.
However, excessive ROS production causes oxidative damage and cell death. Many
pharmacological compounds exert their effects on cell cycle progression by changing
intracellular redox state and in many cases cause oxidative damage leading to drug
cytotoxicity. Appropriate measurement of intracellular ROS levels during cell cycle
progression is therefore crucial in understanding redox-regulation of cell function and drug …
However, excessive ROS production causes oxidative damage and cell death. Many
pharmacological compounds exert their effects on cell cycle progression by changing
intracellular redox state and in many cases cause oxidative damage leading to drug
cytotoxicity. Appropriate measurement of intracellular ROS levels during cell cycle
progression is therefore crucial in understanding redox-regulation of cell function and drug …