Differential expression of CXCR3 and CCR6 on CD4+ T-lymphocytes with distinct memory phenotypes characterizes tuberculosis-associated immune reconstitution …
PS Silveira-Mattos, G Narendran, K Akrami… - Scientific reports, 2019 - nature.com
PS Silveira-Mattos, G Narendran, K Akrami, KF Fukutani, S Anbalagan, K Nayak…
Scientific reports, 2019•nature.comImmune reconstitution inflammatory syndrome (IRIS) occurs in up to 40% of individuals co-
infected with pulmonary tuberculosis (PTB) and HIV, primarily upon antiretroviral therapy
(ART) initiation. Phenotypic changes in T-cells during TB-IRIS and their relationship with
systemic inflammation are not fully understood. In this prospective cohort study, we followed
48 HIV-positive patients with PTB from South India before and after ART initiation, examining
T-lymphocyte subsets and inflammatory biomarkers in peripheral blood. Quantification of …
infected with pulmonary tuberculosis (PTB) and HIV, primarily upon antiretroviral therapy
(ART) initiation. Phenotypic changes in T-cells during TB-IRIS and their relationship with
systemic inflammation are not fully understood. In this prospective cohort study, we followed
48 HIV-positive patients with PTB from South India before and after ART initiation, examining
T-lymphocyte subsets and inflammatory biomarkers in peripheral blood. Quantification of …
Abstract
Immune reconstitution inflammatory syndrome (IRIS) occurs in up to 40% of individuals co-infected with pulmonary tuberculosis (PTB) and HIV, primarily upon antiretroviral therapy (ART) initiation. Phenotypic changes in T-cells during TB-IRIS and their relationship with systemic inflammation are not fully understood. In this prospective cohort study, we followed 48 HIV-positive patients with PTB from South India before and after ART initiation, examining T-lymphocyte subsets and inflammatory biomarkers in peripheral blood. Quantification of naïve (CD27+CD45RO−) as well as effector memory CD4+ T cells (CD27−CD45RO+) at weeks 2–6 after ART initiation could distinguish TB-IRIS from non-IRIS individuals. Additional analyses revealed that ART reconstituted different quantities of CD4+ T lymphocyte subsets with preferential expansion of CXCR3+ CCR6− cells in TB-IRIS patients. Moreover, there was an expansion and functional restoration of central memory (CD27+CD45RO+) CXCR3+CCR6− CD4+ lymphocytes and corresponding cytokines, with reduction in CXCR3−CCR6+ cells after ART initiation only in those who developed TB-IRIS. Together, these observations trace a detailed picture of CD4+ T cell subsets tightly associated with IRIS, which may serve as targets for prophylactic and/or therapeutic interventions in the future.
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